Seed transmission of <i>Carlavirus vignae</i> (<i>Cowpea mild mottle virus</i>): a hidden driver of veinal necrosis and bud blight disease in soybean (<i>Glycine max</i>) in India.

ABSTRACT: A comprehensive investigation was conducted to determine the seed transmission potential of cowpea mild mottle virus (CPMMV) associated with veinal necrosis and bud blight (VNB) disease in soybean (Glycine max) under Indian agroecological conditions during 2024 at ICAR-IARI, New Delhi. Seeds were collected from two CPMMV infected soybean genotypes, Asb-114 and AMS-2022-1 and mechanically sap inoculated cowpea genotypes Arka Samrudhi and Arka Suman. Serological testing of soybean and cowpea seeds using DAC-ELISA did not detect the virus with low absorbance values whereas RT-PCR with coat protein (CP)-specific primers confirmed the presence of CPMMV in whole seed, seed coat, cotyledons, and embryo with the amplification of 867 bp region. Grow out assays demonstrated the vertical trans

SUBMITTER: Bhagwatkar DN 

PROVIDER: S-EPMC12479549 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature