Hypoxia-drives reversible cell cycle arrest in lung cancer cells via modulation of cellular redox and gene expression.

ABSTRACT: Hypoxia is a well-recognized clinical feature of solid tumors, including lung cancer, and is associated with poor prognosis due to its role in promoting resistance to chemotherapy and radiotherapy. To investigate the cellular consequences of hypoxia, we cultured A549 lung adenocarcinoma cells under 1% O₂ and examined their growth, cell cycle distribution, and redox status. Hypoxia significantly reduces cell proliferation and induced G1 phase cell cycle arrest, suggesting a cytostatic effect. Biochemical analysis showed a 2.64- and 2.04-fold increase in total and mitochondrial reactive oxygen species (ROS) levels, respectively, along with an elevated total thiol levels under hypoxic conditions compared to normoxia. To assess the reversibility of the hypoxic response, we performed

SUBMITTER: Maurya DK 

PROVIDER: S-EPMC12479581 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature