Implications of Endogenous Small Regulatory RNAs Survey in Mollusks on Gene Silencing Approaches.

ABSTRACT: Mollusks are an abundant group of animals, with many ecologically and economically important members that are phylogenetically distinct from nearly all genetic model organisms. This study provides a clade-wide evaluation of small RNA biogenesis pathways, with emphasis on the eastern oyster, Crassostrea virginica. A more thorough characterization of these molecules supports rationale design of RNA interference (RNAi) approaches for manipulation of mollusk genetics. Like other animal groups, mollusks have conserved microRNAs (miRNAs), with some shared with ecdysozoans and deuterostomes; however, there was no evidence of a dedicated endogenous small-interfering RNA (siRNA) pathway. These findings suggest that alternatives to long double-stranded RNA (dsRNA)-mediated knockdown, specifically sh

SUBMITTER: Von Eiff C 

PROVIDER: S-EPMC12479628 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature