Generalizability of Enzyme-Based Isolation Approach for Extracellular Vesicles From Traditional Medicinal Plants.

ABSTRACT: Extracellular vesicles (EVs) from plants, particularly traditional medicinal plants, have garnered therapeutic interest. However, therapeutic utility requires establishing effective extraction and purification protocols for manufacturing. Here, we systemically optimized an enzyme-based EV extraction method and demonstrated the generalizability with different parts from seventeen medicinal plants, fresh, frozen or dried. The enzymatic pulping process (henceforth denoted as Phyto-EVpure) of fresh ginger, Morus alba leaves and Isatis indigotica Fort. roots improved EV yield and purity over grinding. Ginger EVs processed via Phyto-EVpure preserved bioactivities and conferred hepatoprotection in a non-alcoholic fatty liver disease model in vitro and in vivo. Phyto-EVpure efficient

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12479721 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature