MiR-340 reverses chemotherapy resistance in colon cancer via the PDCD4/WNT/β-catenin signalling pathway.

ABSTRACT: The silencing of miR-340 has been implicated in the progression and migratory capacity of colorectal cancer (CRC); however, its role in modulating oxaliplatin-induced chemotherapy resistance in CRC remains largely unexplored. In this study, we investigated the influence of miR-340 on oxaliplatin resistance in CRC and elucidated the underlying molecular mechanisms. Oxaliplatin-resistant HCT116 (HCT116-OxR) and CT26 (CT26-OxR) cells were induced with 20 ng/mL oxaliplatin for approximately 7 months. The viability of the treated cells was detected by a CCK-8 assay. Apoptosis was determined via flow cytometry. The mRNA and protein expression levels of resistance-related genes were assessed via qRT‒PCR and western blotting (WB), respectively. P-gp expression in HCT116-OxR and HCT116-OxR-miR-506

SUBMITTER: Xu H 

PROVIDER: S-EPMC12480034 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature