Cell division cycle 25 C (CDC25C) mediates cell-cycle progression and immune evasion in glioma.

ABSTRACT:

Background

Gliomas are highly aggressive brain tumors with poor outcomes. CDC25C, a phosphatase that activates the CDK1-Cyclin B1 complex at the G₂/M checkpoint, is implicated in several cancers but its role in glioma progression and tumor immunity remains unclear.

Methods

We integrated bulk transcriptomic datasets (TCGA, CGGA, GEO) and single-cell RNA sequencing data to evaluate CDC25C expression, prognostic significance, and associated pathways in gliomas. Immune cell infiltration and checkpoint gene correlations were assessed via computational deconvolution. Additionally, U87MG glioma cells were transfected with siRNAs targeting CDC25C to achieve transient knockdown. Proliferation was measured by EdU incorporation, cell-cycle distribution by flow cytometry, and expression

SUBMITTER: Qiao A 

PROVIDER: S-EPMC12480179 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature