DNMT3A-R882 mutation promotes acute myeloid leukemia progression by recruiting E2H2 to inhibit circKCNQ5 methylation.

ABSTRACT:

Background

Patients with acute myeloid leukemia (AML) harboring the DNMT3A-R882 mutation (DR882MUT) are often more difficult to treat and more likely to relapse. However, whether DR882MUT modulates AML progression via circRNAs remains unknown.

Methods

Bioinformatics was used to assess the aberrant expression of circRNAs associated with AML. The DR882MUT cell line model was established. circRNA expression in AML cells treated with DR882MUT was analyzed using RT-qPCR. The CCK-8 assay and flow cytometry were used to measure cell proliferation and apoptosis in DR882MUT AML cells after transfection with si-circKCNQ5. The regulatory mechanism of DR882MUT in circKCNQ5 transcription was studied by bisulfite sequencing and ChIP assays.

Results

Bioinformatics analysis revealed

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12480219 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature