Single-cell RNA sequencing reveals NFATc1-mediated regulatory mechanisms of vascular smooth muscle cell osteogenic transformation in the osteosarcoma microenvironment.

ABSTRACT:

Background

Osteosarcoma is the most common primary malignant tumor of bone and occurs predominantly in children and adolescents during periods of accelerated skeletal growth. Within the tumor microenvironment (TME), vascular smooth muscle cells (VSMCs) may acquire osteogenic characteristics. Nuclear factor of activated T cells c1 (NFATc1) is central to skeletal biology, yet its behavior in VSMCs in osteosarcoma remains insufficiently defined.

Methods

We profiled fresh osteosarcoma tissues by single-cell RNA sequencing on the 10x Genomics Chromium platform. Mechanical and enzymatic dissociation yielded single-cell suspensions; viable cells (> 85%) were enriched; libraries were prepared with Single Cell 3' v3 reagents and sequenced on NovaSeq 6000. Reads were processed with Ce

SUBMITTER: Yi X 

PROVIDER: S-EPMC12480290 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature