DNA TOP1α coordinates anther morphogenesis and fertility through gene regulatory networks in arabidopsis.

ABSTRACT: DNA topoisomerase 1 alpha (TOP1α) plays a critical role in plant development, yet its specific involvement in male reproduction is not well understood. Here, we show that TOP1α is essential for Arabidopsis anther development, and its absence leads to severe morphological and functional defects. The top1α1 mutant displays pleiotropic phenotypes, including early flowering and twisted petals, most notably the loss of two microsporangia. This loss stems from disrupted cell division in the L2 layer. Cytological analyses reveal aberrant pollen wall formation and reduced fertility in the mutant. RNA-seq data from top1α1 mutants identify over 2,000 differentially expressed genes, with significant downregulation of key anther development regulators such as SPL, AMS, and MS1, alongside genes involve

SUBMITTER: Elesawi IE 

PROVIDER: S-EPMC12480461 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature