Maternal origins and genetic diversity of Sabahan swamp buffalo using mitochondrial cytochrome b gene.

ABSTRACT: The swamp buffalo (Bubalus bubalis carabanensis) plays a crucial role in agriculture across Southeast Asia but is increasingly underutilized in regions like Sabah, Malaysia. This study presents the first comprehensive statewide genetic assessment of Sabahan swamp buffalo using mitochondrial cytochrome b (cytb) gene sequences to investigate maternal origins and assess genetic diversity. Blood samples were collected from 211 individuals across eight locations in Sabah. DNA extraction and PCR amplification successfully yielded 198 high-quality cytb sequences. Comparative analysis with 1745 publicly available sequences revealed that 197 Sabahan buffalo belong to the SA1 haplogroup, which is common across Southeast Asia. Only four haplotypes were identified, with one dominant haplotype found in

SUBMITTER: Brocklebank JW 

PROVIDER: S-EPMC12480487 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature