Comparative analysis of illumina and oxford nanopore sequencing platforms for 16S rRNA profiling of respiratory microbial communities.

ABSTRACT: The respiratory microbiome plays a crucial role in health and disease, necessitating accurate characterization through high-throughput sequencing technologies. This study provides a comparative analysis of Illumina NextSeq and Oxford Nanopore Technologies (ONT) sequencing platforms for 16 S rRNA profiling of respiratory microbial communities. Illumina sequencing, known for its high accuracy and short-read lengths (~ 300 bp), is widely used for genus-level microbial classification but struggles with species-level resolution due to its limited read length. In contrast, ONT generates full-length 16 S rRNA reads (~ 1,500 bp), enabling higher taxonomic resolution but historically exhibiting higher error rates (5-15%). Analysis of alpha and beta diversity indicated that Illumina captured greater

SUBMITTER: Macip G 

PROVIDER: S-EPMC12480654 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature