Infiltrating macrophages replace Kupffer cells and play diverse roles in severe alcohol-associated hepatitis.

ABSTRACT: Patients with alcohol-associated cirrhosis (AC) may develop severe alcohol-associated hepatitis (sAH), a disease with high short-term mortality. Our previous studies demonstrated that sAH, but not AC livers, are infiltrated with a high number of self-sustaining IL-8⁺ neutrophils that likely drive the transition from AC to sAH. Monocyte-derived macrophages (MoMFs) also infiltrate the liver in sAH, but their roles remain largely obscure. In the present study, we characterized liver macrophages in human liver explants from sAH and AC patients. Our data revealed a marked reduction in Kupffer cells, whereas MoMFs were increased in sAH and AC. Single-cell RNA-Seq analyses revealed several populations in both AC and sAH, including C1Q⁺, S100A8⁺, APOE⁺,

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12480828 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature