Isoleucyl-tRNA synthetase depletion reveals vulnerabilities in Mycobacterium abscessus and Mycobacterium marinum.

ABSTRACT: Mycobacterium abscessus and Mycobacterium marinum are nontuberculous mycobacteria that pose significant challenges due to their high drug resistance and persistence in hostile host environments. Aminoacyl-tRNA synthetases, such as isoleucyl-tRNA synthetase (IleRS), are crucial for protein synthesis and represent promising targets for antimicrobial development. This study investigates the role of IleRS in mycobacterial growth, metabolism, and pathogenesis using conditional gene silencing combined with microbiological, metabolomic, and transcriptomic analyses. Our findings indicate that IleRS is essential for mycobacterial growth and survival during infection. Depletion of IleRS disrupts branched-chain amino acid and pantothenate biosynthesis, leading to metabolic vulnerabilities and impaire

SUBMITTER: Luo D 

PROVIDER: S-EPMC12480861 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature