A cancer-associated TP53 synonymous mutation induces synthesis of the p53 isoform p53/47.

ABSTRACT:

Background

Synonymous mutations (SMs) change the mRNA nucleotide sequences without altering the corresponding amino acid sequence and are usually overlooked due to their perceived lack of influence on protein function. However, emerging reports suggest that SMs play a significant role in disease development and progression.

Methods

Whole exome sequencing, RNA-sequencing, and droplet digital PCR were performed to identify the SMs from the malignant glioma patients. MutaRNA was used to predict the effect of SMs on RNA structure in silico. SHAPE-MaP was performed to probe and assess the effect of SMs on RNA structure in-cellulo.

Results

Here, we report that a Cancer-Associated SM in TP53 codon valine 203 (CASM203) results in the induction of the alternative translation i

SUBMITTER: Sajwan R 

PROVIDER: S-EPMC12480914 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature