Impact of pulse duration on cardiac electroporation: nanosecond pulses enhance cardiomyocyte selectivity and promote a Raman-detected shift towards apoptotic cell death.

ABSTRACT:

Aims

Pulsed field ablation (PFA), a cardiac ablation technique using microsecond pulsed electric fields (µsPEF), is widely used in clinical settings, while nanosecond pulsed electric fields (nsPEF) have recently entered clinical trials. Selective ablation of cardiomyocytes over endothelial cells is critical to prevent adverse remodelling, arrhythmias, and thrombosis, yet comparative data on nsPEF vs. µsPEF remain limited. This study investigates the cytotoxic effects and cell death mechanisms induced by nsPEF and µsPEF in cardiac and endothelial cells.

Methods and results

Human cardiomyocytes and endothelial cells were exposed to varying electric field intensities with nsPEF and µsPEF using custom-built automated setup to assess permeabilization and cell death. Raman spectro

SUBMITTER: Sowa PW 

PROVIDER: S-EPMC12481159 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature