Viable cell sorting by fluidized bed centrifugation enables novel cultivation strategies.

ABSTRACT: In biopharmaceutical manufacturing, continuous perfusion cultivation enables high space-time yields and increased plant utilization, which are critical targets for modern upstream process intensification. However, filter-based cell retention devices, utilized in these processes, have significant disadvantages: Significant sieving effects and the risk of filter blockage alongside the retention of harmful substances and non-viable cells, represent a major challenge and often reduce the viability of the culture. To enable the next-generation of continuous processes, novel cell retention strategies are required. Therefore, the aim of this study was to develop an approach for large-scale sorting of viable and non-viable cells and to investigate its applicability for novel continuous cultivation

SUBMITTER: Saballus M 

PROVIDER: S-EPMC12481202 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature