DNA aggregation and resolubilization in the presence of polyamines probed at the single molecule level using nanopores.

ABSTRACT: The compaction and decompaction of double-stranded DNA play a crucial role in regulating gene expression. We use solid-state nanopores to study, at the single-molecule level, the translocation of DNA chains in relation to the macroscopic phase transition induced by polyamines. To prevent nonspecific interactions between the nanopore surface and DNA, nanopores were passivated, and experiments were performed in high salt conditions. We first determine the translocation dynamics of DNA. The event frequency varies exponentially with the applied voltage and linearly with the polyelectrolyte concentration. The translocation time decreases exponentially with applied voltage. Addition of polyamine to a DNA solution leads to DNA aggregation and subsequent phase separation; a macroscopic DNA-rich an

SUBMITTER: Cai Y 

PROVIDER: S-EPMC12481218 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature