Coordinated regulation of propionyl-CoA carboxylase subunits drives precursor flux optimization in spinosad production.

ABSTRACT: Spinosad, a natural agricultural pesticide produced by Saccharopolyspora spinosa, has gained global attention owing to its potent insecticidal properties and environmental compatibility. To enhance spinosad biosynthesis, the propionyl-CoA carboxylase (PCC) complex responsible for precursor synthesis was investigated in this study. Six engineered strains were constructed using clustered regularly interspaced short palindromic repeats interference (CRISPRi) and overexpression strategies to systematically regulate the core PCC subunits encoded by pccA, pccB1, and pccB2. Upregulation of these genes enhanced PCC activity, improved energy and precursor utilization, and activated metabolic pathways, ultimately leading to increased spinosad production. Contrary to expectations, CRISPRi-medi

SUBMITTER: Xia Z 

PROVIDER: S-EPMC12481512 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature