A Microfluidic Platform Integrating Dielectrophoretic Concentration and Impedimetric Sensing for Rapid <i>Salmonella choleraesuis</i> Detection.

ABSTRACT: Fast and sensitive detection of foodborne bacteria, such as Salmonella, is essential for food safety and public health control. This study constructed a microfluidic device integrating a top-bottom opposite electrode pair for performing positive dielectrophoresis (pDEP) and electrochemical impedance spectroscopy (EIS). The immunomagnetic beads (IMBs) modified with anti-Salmonella antibodies were spiked in Salmonella enterica serovar Choleraesuis (SC)-containing samples for 30 min to form IMBs@SC complexes, and they were separated by a magnet, which can prevent the sample matrix from contaminating the EIS detectors. After applying the 50 kHz/7.5 V_pp pDEP for 3 min to collect the IMBs@SC complexes in the microholes of the working electrode

SUBMITTER: Police Patil AV 

PROVIDER: S-EPMC12481548 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature