Integrative Genotyping and Analysis of Canine Structural Variation Using Long-read and Short-read Data.

ABSTRACT: Structural variation makes an important contribution to canine evolution and phenotypic differences. Although recent advances in long-read sequencing have enabled the generation of multiple canine genome assemblies, most prior analyses of structural variation have relied on short-read sequencing. To offer a more complete assessment of structural variation in canines, we performed an integrative analysis of structural variants present in 12 canine samples with available long-read and short-read sequencing data along with genome assemblies. Use of long-reads permits the discovery of heterozygous variation that is absent in existing haploid assembly representations while offering a marked increase in the ability to identify insertion variants relative to short-read approaches. Examination of

SUBMITTER: Schall PZ 

PROVIDER: S-EPMC12481690 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature