OligoY pipeline for full Y chromosome painting.

ABSTRACT: The Y chromosome's unique structure poses challenges for cytogenetic studies, especially in designing probes for FISH Oligopaint labeling experiments (Fluorescence in situ hybridization). The standard protocol for designing probes for these experiments discards repetitive sequences to avoid off-target hybridization. Given the highly repetitive nature of the Y chromosome, assemblies often remain fragmented, leaving significant regions incompletely sequenced, unprobed, and poorly understood. Among these, the remaining nonrepetitive sequences are usually insufficient to design probes and efficiently perform FISH Oligopaint assays, since they do not cover most regions of the chromosome. This limitation hinders comprehensive cytogenetic studies, which are crucial not only for understanding the

SUBMITTER: Almeida I 

PROVIDER: S-EPMC12481694 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature