FGL1-mediated lymph node metastasis in stage T1 non-small cell lung cancer: therapeutic targeting.

ABSTRACT:

Background

Approximately 30% of patients with stage T1 non-small cell lung cancer (NSCLC) have mediastinal (N2) lymph node metastasis; however, the underlying mechanism remains unclear.

Methods

The cells likely mediating N2 lymph node metastasis in T1 NSCLC were identified by single-cell sequencing. The expression and function of the main functional gene high fibrinogen-like protein 1 (FGL1) in this cell subgroup were analyzed by single-cell analysis. Transcriptome sequencing, metabolome sequencing, and mass spectrometry combined with in vitro and in vivo experiments were conducted, and therapeutic validation was performed using shFGL1_AAV9 and shFGL1_AAV6.

Results

A novel cell subgroup characterized by FGL1 expression was identified (CCNE1(+) cells). FGL1 expression

SUBMITTER: Tang XY 

PROVIDER: S-EPMC12481761 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature