The impact of altered intestinal microbiota on intestinal immune function after acute exhaustive exercise in mice.

ABSTRACT:

Purpose

Long-term training or intense exercise alters gut microbiota. This study aimed to determine the effects of microbiota on colonic permeability and immune function in mice subjected to acute exhaustive exercise.

Methods

C57BL/6 mice were randomly divided into the blank control (C), no exercise experience (NE), under a training protocol (E), phosphate-buffered saline (PBS) transplantation (PT), and fecal microbiota transplantation (FMT) groups. The E group underwent 14 weeks of moderate intensity training. At the end of the 14th week, fecal suspensions were prepared from mice in Group E and transplanted into Group FMT via enema, while Group PT received PBS enemas twice daily for 7 days. Prior to transplantation, both Groups PT and FMT were gavaged with antibiotics for 7

SUBMITTER: Yang G 

PROVIDER: S-EPMC12481770 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature