SURROGATE SELECTION OVERSAMPLES EXPANDED T CELL CLONOTYPES.

ABSTRACT: Surrogate selection is an experimental design that without sequencing any DNA can restrict a sample of cells to those carrying certain genomic mutations. In immunological disease studies, this design may provide a relatively easy approach to enrich a lymphocyte sample with cells relevant to the disease response because the emergence of neutral mutations associates with the proliferation history of clonal subpopulations. A statistical analysis of clonotype sizes provides a structured, quantitative perspective on this useful property of surrogate selection. Our model specification couples within-clonotype birth-death processes with an exchangeable model across clonotypes. Beyond enrichment questions about the surrogate selection design, our framework enables a study of sampling properties of

SUBMITTER: Yu P 

PROVIDER: S-EPMC12481847 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature