Pde5a deficiency prevents diet-induced obesity via adipose cAMP-PKA activation enhancing fat browning.

ABSTRACT:

Objective

Cyclic nucleotides are central regulators of adipogenesis and adaptive thermogenesis, with their intracellular concentrations tightly controlled by phosphodiesterases (PDEs). Among them, phosphodiesterase type 5 (PDE5A) regulates cyclic guanosine monophosphate (cGMP) turnover in adipocytes. Although PDE5A inhibition has been explored in diabetes, its role in systemic metabolism remains poorly defined.

Methods

We employed different Pde5a knockout mouse models to investigate the impact of PDE5A deficiency on adipose tissue biology and whole-body energy homeostasis. Phenotypic, histological, and metabolic assessments were performed under chow and high-fat diet conditions, with a focus on thermogenic activation, hepatic lipid accumulation, and glucose metabolism.

Re

SUBMITTER: Campolo F 

PROVIDER: S-EPMC12481927 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature