Dynamic hydrogel mechanics in organoid engineering: From matrix design to translational paradigms.

ABSTRACT: The extracellular matrix (ECM) serves as a dynamic biomechanical regulator of cellular behavior, yet conventional 3D culture systems, such as Matrigel, lack the spatiotemporal control required to dissect mechanotransductive mechanisms in organoids. This review systematically explores the synthesis of mechanically tunable hydrogels-spanning stiffness and viscoelasticity-and their transformative applications in organoid research. By integrating natural, synthetic, and hybrid polymers, these hydrogels enable precise recapitulation of tissue-specific ECM mechanics, overcoming limitations of batch variability and static properties. We categorize hydrogel design strategies, emphasizing crosslinking paradigms (physical vs. chemical) and dynamic bond engineering, which permit real-time modulation

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12481939 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature