SUZ12 knockdown restrains the proliferation, migration, and invasion of oral tongue squamous cell carcinoma through inhibiting DNMT1-mediated ZNF582 promoter methylation.

ABSTRACT:

Background

Suppressor of zest 12 (SUZ12) upregulation is related to cervical node metastasis in oral tongue squamous cell carcinoma (OTSCC). This study explored the roles and mechanism of SUZ12 in OTSCC progression.

Methods

SUZ12 protein levels in OTSCC tissues and cell lines were determined using immunohistochemistry assay and Western blot analysis. SUZ12 was overexpressed or silenced through transfection of Overexpression plasmids or small interfering RNA targeting SUZ12 were transfected into CAL27 and SCC9 cells to achieve SUZ12 overexpression or knockdown. Subsequently, cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin and Vimentin) were determined. Zinc finger protein 582 (ZNF582) promoter methylation wa

SUBMITTER: Kong X 

PROVIDER: S-EPMC12482123 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature