Potential for prolonged replication of common acute respiratory viruses in air-liquid interface cultures of primary human airway cells.

ABSTRACT: A previous study provides clinical evidence that extended respiratory virus detection can occur in healthy pediatric individuals. However, it remains unclear how long respiratory viruses can survive in the human respiratory tissue. In this study, the replication potential of common respiratory viruses was evaluated using air-liquid interface (ALI) cultures of primary human respiratory epithelial cells. The findings demonstrate that most respiratory viruses can replicate for approximately 100 days in ALI cultures, with some showing prolonged replication for up to 150-200 days. In contrast, influenza and respiratory DNA viruses showed shorter replication periods likely due to virus-induced cell death. Even during the late phase, some samples continued to support viral replication in re-infec

SUBMITTER: Kawase M 

PROVIDER: S-EPMC12482148 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature