Using BONCAT to dissect the proteome of <i>S. aureus</i> persisters.

ABSTRACT: Bacterial persisters are a subpopulation of cells that exhibit a transient non-susceptible phenotype in the presence of bactericidal antibiotic concentrations. This phenotype can lead to the survival and regrowth of bacteria after treatment, resulting in relapse of infections. It is also a contributing factor to antibacterial resistance. Multiple processes are believed to cause persister formation; however, identifying the proteins expressed during the induction of persistence is challenging because the persister state is rare, transient, and does not result in genetic changes. In this study, we used Bio-Orthogonal Non-Canonical Amino Acid Tagging (BONCAT) to label and retrieve the proteome expressed during persistence and recovery for two strains of Staphylococcus aureus exposed to

SUBMITTER: George Matlalcuatzi EDC 

PROVIDER: S-EPMC12482189 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature