EefR mutations drive sanguinarine resistance by activating cryptic multidrug efflux pumps in AcrB-Null <i>Escherichia coli</i>.

ABSTRACT: Antimicrobial resistance is one of the greatest threats to global health. A thorough comprehension of the underlying processes through which bacteria evolve the ability to withstand diverse stressors is vital for the development of efficacious strategies to address this challenge. This study aimed to elucidate the mechanisms underlying the adaptation of an acrB-deficient strain of Escherichia coli ATCC 35218 (35218m) to sanguinarine (SAN), the main component of phytobiotic derived from Macleaya cordata. Analysis of the in vitro-selected SAN-resistant clones of strain 35218m led to the identification of a functionally uncharacterized TetR regulator, EefR. The regulator represses its own expression and that of four neighboring genes forming the eefRABCD ope

SUBMITTER: Shen HJ 

PROVIDER: S-EPMC12482432 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature