Pathological protection and molecular regulation of cystatin B in Nile tilapia (<i>Oreochromis niloticus</i>) bacterial disease.

ABSTRACT: Cystatin B (CSTB) is an endogenous cysteine protease inhibitor that plays a critical role in the modulation of numerous biological processes, involving immune responses, apoptosis, and inflammation in mammals. However, its immunological functions in teleost, particularly Nile tilapia (Oreochromis niloticus) remain unclear. In the present study, the CSTB was cloned and characterized from Nile tilapia (On-CSTB) and its role in bacterial infection was revealed. The On-CSTB open reading frame is 300 bp encoding 99 amino acids that contains a conserved cystatin domain. Multiple sequence alignment analysis revealed that On-CSTB share over 75% identity with fish lineages, and 45% identity with mammals. qPCR analysis showed that On-CSTB is widely expressed across variou

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12482433 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature