STALARD: Selective Target Amplification for Low-Abundance RNA Detection.

ABSTRACT:

Background

Accurate quantification of RNA isoforms is critical for understanding gene regulation. However, conventional reverse transcription-quantitative real-time PCR (RT-qPCR) has limited sensitivity for low-abundance transcript isoforms, as quantification cycle (Cq) values above 30 are often considered unreliable. While transcriptome-wide analyses can address this limitation, they require costly deep sequencing and complex bioinformatics. Moreover, isoform-specific qPCR is often confounded by differential primer efficiency when comparing similar transcripts.

Results

To overcome the sensitivity and amplification bias limitations of conventional RT-qPCR for detecting known low-abundance and alternatively spliced transcripts, we developed STALARD (Selective Target Amplifica

SUBMITTER: Jeong D 

PROVIDER: S-EPMC12482509 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature