Photoperiodic responsiveness in the DNA methylation and gene expression in the hypothalamus of ovariectomized and estradiol-treated ewes.

ABSTRACT:

Background

Sheep are short-day breeder, and their reproductvie activity can be activated by short photoperiods, but the regulatory mechanism remains unclear. In this study, hypothalamic tissues were collected from ovariectomized and estradiol-treated (OVX + E₂) Sunite ewes exposed to artificial short-day (SP) and long-day (LP) photoperiods. Integrated whole-genome DNA methylation and transcriptomic analyses were performed to elucidate the regulatory mechanisms underlying photoperiod-mediated seasonal reproduction.

Results

The DNA methylation profiles of the hypothalamus were examined, and whole-genome single-base resolution methylome maps of the sheep hypothalamus were generated across seven time points under various photoperiod treatments. The results revealed th

SUBMITTER: He X 

PROVIDER: S-EPMC12482692 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature