Comprehensive profiling of ribo-seq detected small sequences in yeast reveals robust conservation patterns and their potential mechanisms of origin.

ABSTRACT:

Background

In the budding yeast Saccharomyces cerevisiae, the widespread adoption of ribosome profiling technology has allowed the discovery of evidence of transcription and translation for thousands of small proteins or microproteins whose importance was once disregarded. Both conserved and evolutionarily short-lived microproteins have demonstrated relevant involvement in biological functions. However, sequences exist in a broad spectrum of conservation. Here, we tested whether these small proteins in yeast detected by ribosome profiling technology have different properties across their levels of conservation, and how do these properties compare with the canonical small protein-coding sequences.

Results

Here, we applied a phylostratigraphic approach to peptides encoded by s

SUBMITTER: Reyes Loaiciga C 

PROVIDER: S-EPMC12482700 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature