Uncovering key anti-inflammatory constituents and mechanism of Wuwei Xiaodu Decoction by a combined strategy of phytochemistry, AIDD, network pharmacology, and in vitro and in vivo assay.

ABSTRACT:

Background

Wuwei Xiaodu decoction (WXD), a renowned prescription in traditional Chinese medicine, is widely used to treat various skin diseases, including atopic dermatitis. However, the bioactive constituents and anti-inflammatory mechanisms remain unclear.

Purpose

The study was designed to investigate the most important bioactive components and the anti-inflammatory molecular mechanisms of WXD that have not been fully elucidated.

Materials and methods

The mixed herbs of WXD were extracted by different solvents, including ethyl-acetate (EA) and petroleum-ether (PE). HPLC/LC-MS and GC-MS were performed to identify the chemical composition of WXD-EA and WXD-PE, respectively. The RAW264.7 cell line was used to assess the inflammatory responses, while CCK8 assay was empl

SUBMITTER: Li B 

PROVIDER: S-EPMC12482766 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature