Modification of adipose mass by targeting distal enhancers of Ptrf.

ABSTRACT:

Background

Adipose tissue plays a central role in systemically metabolic regulation. Polymerase I and transcript release factor (PTRF) is responsible for caveolae structure formation and plays a crucial role in lipid metabolism.

Results

We investigated the possibility of modifying adipose mass by targeting the enhancers of Ptrf. Single-nucleus transcriptomic analysis revealed that the reduction in fat mass caused by Ptrf knockout is associated with impaired adipogenesis and differentiation, adipocyte metabolism, nutrient transportation, and altered regulatory network. We demonstrated a closely positive association between Ptrf transcription and adipose mass in mice. Then, circular chromosome conformation capture sequencing and ChIP-seq data of mature adipocytes identified si

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12482881 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature