Transcriptomic insights and feeder-free culturing of porcine expanded potential stem cells from cloned embryos.

ABSTRACT:

Background

Generating expanded potential stem cells from cloned porcine embryos (pEPSCs^NT) represents a notable advancement in regenerative medicine and agricultural biotechnology. However, challenges, including low derivation efficiency, limited understanding of transcriptomic features, and unknown feasibility of culturing under feeder-free conditions, remain. This study aimed to generate pEPSCs using blastocysts derived from parthenogenetic activation, in vitro fertilization, and somatic cell nuclear transfer (SCNT) using a modified culture system.

Methods

We derived pEPSC^NT lines using an optimized culture system. We characterized the pEPSC lines from all three origins by analyzing pluripotent marker expression, performing karyotyping, and assessing

SUBMITTER: Cai L 

PROVIDER: S-EPMC12482889 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature