Cross-species rescue reveals sequence requirements for a rapidly evolving intrinsically disordered region.

ABSTRACT: Many proteins contain intrinsically disordered regions (IDRs) that are essential for their function but do not adopt a stable structure; instead, they exist as an ensemble of conformations. Because these regions lack fixed structural constraints, traditional structure-based and alignment-based approaches are often ineffective for studying their sequence-function relationships. Here, we present an approach that combines molecular evolution with genetic complementation to extract functional sequence features of an IDR. We use the budding yeast RNA-binding protein Rim4 as a model system. Rim4 is required for sporulation, and its IDR facilitates its dual role as a translational activator and repressor. Notably, Rim4's IDR supports assembly into an SDS-resistant amyloid-like form, which is requ

SUBMITTER: Park SO 

PROVIDER: S-EPMC12483275 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature