Molecular signatures of alveolar type II cell differentiation in acute respiratory distress syndrome.

ABSTRACT:

Background

Acute respiratory distress syndrome (ARDS) is a life-threatening lung condition with high morbidity and mortality.

Methods

We analyzed publicly available single-cell transcriptomic and microarray datasets from murine ARDS models to characterize AT2 cell differentiation trajectories following lipopolysaccharide (LPS)-induced injury. Computational mapping of cell "trajectories" revealed distinct gene expression signatures associated with divergent repair outcomes. These findings were validated in bronchoalveolar lavage fluid (BALF) samples from ARDS patients and in an LPS-induced AT2-fibroblast co-culture model. Gene expression changes were examined at both the RNA and protein levels, and pathway enrichment analysis was used to explore underlying mechanisms.

Resu

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12483913 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature