Serum matters: human platelet lysate enables physiological modeling of HIV-1 infection in dendritic cells.

ABSTRACT: Monocyte-derived Dendritic Cells (moDC) are fundamentally involved in infectious processes and serve as model cell type for studying immune regulatory functions in vitro. How DCs behave in viral infections is influenced by various intra- and extracellular factors. Thus, working as physiologically as possible is crucially important for gaining mechanistic insights. We and others previously stressed a critical role of DCs in the course and transmission of human immunodeficiency virus (HIV) infection. Up to date, moDCs are differentiated in presence of FCS-based media, comprising undefined factors that might impact cell differentiation behavior and function. While a few studies addressed working under xeno-free conditions enhancing reproducibility and clinical applicability, difference

SUBMITTER: Schweighofer P 

PROVIDER: S-EPMC12484019 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature