Rapid identification of major <i>Mycobacterium</i> species by loop-mediated isothermal amplification assay using novel species-specific genomic targets.

ABSTRACT:

Background

Rapid and precise identification of Mycobacterium species is critical for appropriate clinical management and epidemiological surveillance. However, conventional methods often fail to differentiate closely related nontuberculous mycobacteria (NTM) species, limiting their clinical utility.

Methods

We developed a multiplex loop-mediated isothermal amplification (LAMP) assay targeting newly identified species-specific genomic markers for simultaneous detection of Mycobacterium tuberculosis complex (MTBC) and six clinically important NTM species. Analytical performance was assessed using serial dilutions of bacterial cultures and 36 reference strains. Clinical validation was performed on 52 cultured isolates and 349 sputum samples, compared to GeneXpert

SUBMITTER: Zou Y 

PROVIDER: S-EPMC12484021 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature