Diet gel-based oral drug delivery system for controlled dosing of small molecules for microglia depletion and inducible Cre recombination in mice.

ABSTRACT: Small molecules such as PLX5622 for microglia depletion and tamoxifen for inducible Cre recombination are commonly used in mouse research. Traditional application methods such as drug-infused chow, oral gavage or injections have limitations, including uncontrolled dosing (chow) or risk of injury and/or stress (gavage or injections). Here, to address these issues, we have developed an alternative oral drug delivery system using a gel-based rodent maintenance diet that allows for controlled consumption and adjustment of dosage and is suitable for water-insoluble small molecules. We tested DietGel 93M (93M) infused with PLX5622 (0.8 mg/g and 2.0 mg/g) in the Cx3cr1^gfp/+ retinal microglia reporter mouse and tamoxifen-infused 93M (0.3125 mg/g) in the Rlbp1-CreERT2;Rosa^ai14

SUBMITTER: Jovanovic J 

PROVIDER: S-EPMC12484080 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature