Cloning and high temperature tolerance analysis of the thermal response related gene <i>KcRCB</i> in <i>Karelinia caspia</i> (Pall.) Less.

ABSTRACT:

Introduction

The REGULATOR OF CHLOROPLAST BIOGENESIS (RCB) is a novel protein component in plant temperature signaling that functions by synergizing with HEMERA (HMR) to initiate thermomorphogenesis by stabilizing PHYTOCHROME INTERACTING TRANSCRIPTION FACTOR 4 (PIF4) during the day.

Methods

In this study, we successfully cloned the heat-responsive gene KcRCB from Karelinia caspia, a desert-adapted plant species. KcRCB transcript levels were significantly elevated when the plants were exposed to high temperatures. Furthermore, KcRCB demonstrated differential expression in the Karelinia caspia roots, stems, and leaves, with optimal expression in the leaves. Subsequently, KcRCB transgene was overexpressed in Arabidopsis thaliana and cotton plants to characterize its thermomorph

SUBMITTER: Li W 

PROVIDER: S-EPMC12484188 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature