Generation of novel human anti-OX-40 mAbs endowed with different biological properties as tools for cancer therapy.

ABSTRACT:

Introduction

The second generation of Antibody-based Immunotherapy includes monoclonal antibodies against Immune Checkpoints (ICs), to modulate specific T cell responses against cancer or viruses. We recently generated a large repertoire of fully human antibodies targeting ten different ICs through a novel selection strategy based on the combination of phage libraries on human lymphocytes and next generation sequencing (NGS). Here we generated and tested four novel fully human IgG1 mAbs specific for OX-40, an immunostimulatory receptor expressed on immune cells, which has been shown to be a promising target for immune-based therapeutic strategies.

Methods and results

By ELISA and Biolayer Interferometry we demonstrated that they all specifically bind with high affinity to OX

SUBMITTER: Rapuano Lembo R 

PROVIDER: S-EPMC12484235 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature