Mitochondrial respiratory function in human platelets: Influence of sample preparation, assay buffer, and instrumental platform.

ABSTRACT: Circulating blood cells such as platelets represent a readily available sample type to determine mitochondrial function in humans. Here, we set out to determine the influence of sample preparation, assay buffer composition, and instrumental platform on the respiratory function of platelets isolated from human blood. Approximately 50 mL of whole blood was collected from healthy adults (n = 16) following an overnight (>12 h) fast. Platelets were immediately isolated from whole blood by centrifugation for respirometry. Respiratory function was assayed in intact and permeabilized platelets using an Oxygraph-2K (O2K) high-resolution respirometer in either RPMI or MIR05 (containing 5 mM glucose, 1 mM pyruvate, and 2 mM glutamine), or the participant's own plasma. In addition, respiratory functio

SUBMITTER: Porter C 

PROVIDER: S-EPMC12484291 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature