Comparative evaluation of CD34 immunohistochemistry and hematoxylin and eosin staining for detection of lymphovascular emboli in oral squamous cell carcinoma: a retrospective cross-sectional study in a developing country.

ABSTRACT:

Objectives

The primary objective was to evaluate and compare the sensitivity and specificity of CD34 immunohistochemistry (IHC) versus conventional hematoxylin and eosin (H&E) staining in detecting lymphovascular emboli (LVE) in oral squamous cell carcinoma (OSCC). The secondary objective was to correlate LVE detection with tumor size, grade, perineural invasion (PNI), depth of invasion (DOI), worst pattern of invasion (WPOI), and lymph node involvement, with or without extranodal extension (ENE).

Methodology

A retrospective, cross-sectional comparative analysis based on archival cases from the period of January 2021 to December 2023, in pathology department in a tertiary care hospital in central India. A total of 93 patients diagnosed with OSCC were included. All cases had

SUBMITTER: Hatgaonkar K 

PROVIDER: S-EPMC12484456 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature