Spatiotemporal regulation of endoplasmic reticulum stress visualized by live imaging of bipA mRNA in Aspergillus oryzae.

ABSTRACT: The molecular mechanisms of the endoplasmic reticulum (ER) stress response have been elucidated in detail in many eukaryotic organisms. However, there is still a lack of cellular biological knowledge about the ER stress response in filamentous fungi. In this study, we utilized the MS2 system to analyze the mRNA of bipA, which encodes a typical ER chaperone expressed in response to dithiothreitol (DTT)-induced ER stress in living cells of Aspergillus oryzae. Fluorescence microscopy revealed the temporal changes in the amount of bipA mRNA in the cell with or without DTT. It was also shown that when ER stress was induced with DTT, bipA mRNA expression was observed from the apical to the basal regions of the hyphal cells. Moreover, although some of the ER stress-induced bipA mRNA showed microt

SUBMITTER: Sathongdejwisit P 

PROVIDER: S-EPMC12484549 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature