Escherichia coli phylogeny drives co-amoxiclav resistance through variable expression of TEM-1 beta-lactamase.

ABSTRACT: Co-amoxiclav (amoxicillin and clavulanate) is a commonly used combination antibiotic, with resistance in Escherichia coli associated with increased mortality. The class A beta-lactamase bla_TEM-1 is often carried by resistant E. coli but exhibits high phenotypic heterogeneity, complicating genotype-phenotype predictions. We curated a dataset of n = 377 diverse E. coli isolates where the only acquired beta-lactamase was bla_TEM-1. We generated hybrid assemblies and co-amoxiclav minimum inhibitory concentrations (MICs), and bla_TEM-1 qPCR expression data for a subset (n = 67/377). We first tested whether intrinsic expression of bla_TEM-1 varied between E. coli lineages, for example, from regulatory system differences, which are challenging to genomical

SUBMITTER: Matlock W 

PROVIDER: S-EPMC12484619 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature