Dihydroartemisinin ameliorates renal tubulointerstitial fibrosis in diabetic nephropathy via restoring mitochondrial function via HIF-1α/HO-1 pathway.

ABSTRACT: Dihydroartemisinin (DHA) is a potential therapeutic strategy in multiple diseases, but its effects on Diabetic nephropathy (DN) have been not explored yet. Rats underwent unilateral ureteral obstruction surgery and injected by streptozotocin to induce DN, with treatment by DHA (15 and 30 mg/kg), and Losartan was used as a positive control. Blood glucose, and 24-h urine protein and glucose tolerance were detected to ensure kidney function. Periodic acid Schiff, hematoxylin and eosin and Masson staining were conducted to assess histological changes. Western blot and immunofluorescence were used for examining protein expression. HK-2 cells were subjected to high glucose (HG) exposure. Flow cytometry and Cell counting kit-8 were applied for assessing cell apoptosis and viability. Mitochondrial

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12484724 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature