ABSTRACT: The development of a vaccine against Helicobacter pylori remains a public health priority due to its role in various gastric and non-gastric diseases, compounded by the rise of antibiotic resistance. To address the urgent need for novel interventions, we designed an in-silico self-amplifying RNA (saRNA)-based multi-epitope peptide vaccine targeting critical stages of H. pylori pathogenesis: gastric acid neutralization, bacterial adhesion, and toxin-mediated tissue damage. We employed a comprehensive computational and immunoinformatics pipeline and tools such as Geptop 2.0, VaxiJen, PSORTb v3.0.2, VirulentPred 2.0, BLASTp, TMHMM, and Expasy, IEDB's NetMHCpan, NetMHCIIpan, AutoDock Vina, GROMACS v2024, PSIPRED, I-TASSER, PROSA-web, ERRAT, ClusPro 2.0, iMODS to identify, prioritize and valida
